Although its quite awkwark to talk about steriliation being a third year undergrad, I would like to let people know about the importance of it. As molecular biologists, we have to rely on colony palting and clonning no matter which area of research we go into. The factor underpinning the success behind these techniques is mainly the good output of our lab culture and their subsequent gel results. Even slight contamination can lead to the major error in the experimental interpretation.
Today I learnt about good laboratory practice from Dr Stansfield and also got some useful tips which might be useful for the others:
- Always maintain a concise and clean lab book with which you can talk in your free time!! By stating that I mean always write down everything (i.e. a little spot in one of your gel lanes ) you observe as you may need to interpret that part for your later experimantal steps.
- Always sketch a plan prior to the experiment.
- Never forget to wear gloves while working in the lab.
- Always wash your hand before you enter and leave the lab.
- Write down the lane markers before running a gel as mistake may lead to the repeat of the whole procedure!
- Try to organise your workstation by ordering the tubes, burners, papers around you.
- Try to get the burner going while working with microbial culture.
- Never leave open your pippette tip box or any other tube.
- Never collide the sides of your pippettes with the solution tubes while dealing with them
I will come up with some more tips as I learn through my project.............Have a nice evening!!!!
No comments:
Post a Comment